Real Madrid duo Raphael Varane and Gareth Bale will not face Tottenham Hotspur on Wednesday evening through injury.
The French defender and Welsh winger are among six players unavailable for Madrid’s game against Spurs at Wembley.
Reports had surfaced that Bale would recover in time to face his former club, but he trained alone on Monday at Valdebebas and has now been left out of the travelling squad.
Keeper Keylor Navas, who has missed Madrid’s last three games with an abductor problem – is also ruled out and will be replaced in the starting eleven by Kiko Casilla.
Both Isco and Varane suffered knocks in the 2-1 defeat to Girona on Sunday evening and they trained alone and indoors on Monday morning, with the latter being unable to recover in time to face the English side.
Dani Carvajal and Mateo Kovacic remain still sidelined, meaning Zidane has a selection headache before meeting Mauricio Pochettino’s side.
Madrid and Spurs are level on seven points each in Champions League Group H and a victory for either will guarantee them a place in the knockout stages of the competition.
Having started the season in stuttering form, Madrid had bounced back slightly with wins over Eibar, Getafe, Espanyol and Alaves.
However, their run of successive victories came to an end on Sunday after a shock defeat to Catalan side Girona.
Isco’s opening goal was cancelled out by Cristhian Stuani, before Portu grabbed a winner for the newly promoted La Liga side.
Real Madrid squad: Casilla, Moha, Achraf, Ramos, Nacho, Marcelo, Theo, Vallejo, Kroos, Modric, Casemiro, Asensio, Isco, Marcos Llorente, Ceballos, Ronaldo, Benzema, Lucas Vazquez, Mayoral